4P7V
Structural insights into higher-order assembly and function of the bacterial microcompartment protein PduA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.040, 53.340, 68.120 |
| Unit cell angles | 90.00, 117.64, 90.00 |
Refinement procedure
| Resolution | 60.350 - 1.930 |
| R-factor | 0.2099 |
| Rwork | 0.207 |
| R-free | 0.27054 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.040 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.350 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.050 | 0.363 |
| Number of reflections | 29930 | |
| <I/σ(I)> | 10.7 | 2.4 |
| Completeness [%] | 94.3 | 95.5 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | The reservoir for type II crystals was 1.0 M sodium citrate and 0.1 M Tris at pH 8.5 and the protein used was at 6.3 mg/mg |
