4P7T
Structural insights into higher-order assembly and function of the bacterial microcompartment protein PduA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.240, 93.300, 63.050 |
| Unit cell angles | 90.00, 105.03, 90.00 |
Refinement procedure
| Resolution | 60.890 - 1.720 |
| R-factor | 0.1826 |
| Rwork | 0.180 |
| R-free | 0.23025 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.507 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.890 | 1.760 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.060 | 0.617 |
| Number of reflections | 52279 | |
| <I/σ(I)> | 13.9 | 2.2 |
| Completeness [%] | 99.4 | 98.6 |
| Redundancy | 5.1 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Type I crystals were harvested from protein drops (2.9 mg/ml) equilibrated against a reservoir of 1.3 M sodium citrate tribasic dihydrate, 0.1 M Sodium HEPES, pH 7.9. |
