4P7O
Structure of Escherichia coli PgaB C-terminal domain, P1 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.618, 54.016, 86.460 |
| Unit cell angles | 101.70, 98.30, 90.19 |
Refinement procedure
| Resolution | 41.864 - 1.480 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.25320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p7l |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.075 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1615)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.057 | 0.299 |
| Number of reflections | 126137 | |
| <I/σ(I)> | 11.3 | 2.4 |
| Completeness [%] | 94.1 | 90.4 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 30% PEG5000 MME, 0.2 M ammonium sulfate, 0.1 M MES |
