4P7H

Structure of Human beta-Cardiac Myosin Motor Domain::GFP chimera

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-05-25
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54056
Spacegroup name	P 1
Unit cell lengths	59.690, 97.730, 118.610
Unit cell angles	71.06, 82.67, 75.08

Refinement procedure

Resolution	37.939 - 3.200
R-factor	0.2294
Rwork	0.226
R-free	0.28380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 2MYS 2qle
RMSD bond length	0.002
RMSD bond angle	0.524
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.560	3.370
High resolution limit [Å]	3.200	3.200
Number of reflections	36487
<I/σ(I)>	8.1	1.8
Completeness [%]	90.4	81.2
Redundancy	6.7	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	10% Tacsimate, pH 6.0, 10% glycerol, 14-15% PEG 3350, 0.2 mM MgCL2, and 5 mM TCEP