4P79

Crystal structure of mouse claudin-15

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-08
Detector	RAYONIX MX300HE
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	123.250, 28.403, 58.587
Unit cell angles	90.00, 100.84, 90.00

Refinement procedure

Resolution	38.265 - 2.400
R-factor	0.2242
Rwork	0.221
R-free	0.25270
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.487
Data scaling software	XDS
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.265		2.460
High resolution limit [Å]	2.400	10.720	2.400
Rmerge	0.227	0.048	1.813
Rmeas	0.243	0.051	1.971
Total number of observations	113916
Number of reflections	14967	167	1097
<I/σ(I)>	7.92	28.03	1.33
Completeness [%]	97.7	96.5	98.3
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7.5	293	30% PEG400, 10 mM HEPES-NaOH, 2 mM 2-mercaptoethanol