4P77
Structure of ribB complexed with substrate Ru5P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-06-25 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.408, 74.086, 99.331 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.040 |
| R-factor | 0.17589 |
| Rwork | 0.173 |
| R-free | 0.22356 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p6d |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.310 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.160 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.085 | 0.535 |
| Number of reflections | 26194 | |
| <I/σ(I)> | 19.2 | 3.7 |
| Completeness [%] | 99.4 | 97.8 |
| Redundancy | 6.9 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.1 | 293 | Na2HPO4, PEG3350 |
