4P76

Cellular response to a crystal-forming protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.900
Spacegroup name	P 21 21 2
Unit cell lengths	49.862, 84.426, 118.218
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.354 - 2.900
R-factor	0.17784
Rwork	0.174
R-free	0.24990
RMSD bond length	0.013
RMSD bond angle	1.767
Refinement software	REFMAC (5.8.0069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Rmerge	0.161	0.470
Number of reflections	11052
<I/σ(I)>	19.3	6.7
Completeness [%]	99.8	100
Redundancy	6.3	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		310