4P70
Crystal Structure of Unmodified tRNA Proline (CGG) Bound to Codon CCG on the Ribosome
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97913 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 209.816, 447.388, 619.857 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 187.581 - 3.680 |
| R-factor | 0.2148 |
| Rwork | 0.212 |
| R-free | 0.27160 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.311 |
| Data scaling software | XDS |
| Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 187.581 | 3.630 |
| High resolution limit [Å] | 3.540 | 3.540 |
| Rmerge | 0.339 | 1.440 |
| Number of reflections | 696967 | |
| <I/σ(I)> | 4.97 | 1.07 |
| Completeness [%] | 99.2 | 99.8 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl |
