4P6I
Crystal structure of the Cas1-Cas2 complex from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-09-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.116 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.875, 125.696, 99.306 |
| Unit cell angles | 90.00, 102.74, 90.00 |
Refinement procedure
| Resolution | 62.848 - 2.300 |
| R-factor | 0.2258 |
| Rwork | 0.225 |
| R-free | 0.24510 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.617 |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.850 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 1.090 | |
| Number of reflections | 97929 | |
| <I/σ(I)> | 9.19 | 1 |
| Completeness [%] | 97.2 | 91.21 |
| Redundancy | 3.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 150 mM NaCl, 5% w/v PEG 8000, 100 mM Tris pH 8.0 |
