4P6D

Structure of ribB complexed with PO4 ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-26
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9780
Spacegroup name	P 21 21 21
Unit cell lengths	56.554, 73.597, 95.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.436 - 1.590
R-factor	0.1702
Rwork	0.169
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g57
RMSD bond length	0.006
RMSD bond angle	1.089
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1615))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.680
High resolution limit [Å]	1.590	1.590
Rmerge	0.036	0.197
Number of reflections	52849
<I/σ(I)>	32.1	7.4
Completeness [%]	96.6	93.5
Redundancy	5.4	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	NaH2PO4, PEG3350