4P61
CHICKEN TRIOSEPHOSPHATE ISOMERASE WITH LOOP6 MUTATIONS, V167P AND W168E.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.357, 56.017, 105.782 |
| Unit cell angles | 90.00, 92.83, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.340 |
| R-factor | 0.16574 |
| Rwork | 0.164 |
| R-free | 0.20538 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.059 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.400 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.061 | |
| Number of reflections | 98338 | |
| <I/σ(I)> | 27.7 | |
| Completeness [%] | 95.8 | 88.1 |
| Redundancy | 2.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 26% PEG 3350 and 0.1 M Tris, pH 8.5 |
