4P55

Crystal structure of DNA binding domain of K11 from KSHV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-05
Detector	MAR CCD 165 mm
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	48.467, 48.467, 195.088
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.035 - 2.504
R-factor	0.2123
Rwork	0.210
R-free	0.24840
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.257
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.134	0.468
Number of reflections	9881
<I/σ(I)>	29.55	3.95
Completeness [%]	100.0	100
Redundancy	15.3	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	277	0.04 M Citric acid, 0.1 M BIS-TRIS propane (pH 6.8), 17% PEG3350