4P54
Crystal Structure of the Helicobacter pylori MTAN-D198N mutant with 5'-methylthioadenosine in the active site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.078 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 81.207, 81.207, 67.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.730 - 1.650 |
| R-factor | 0.1597 |
| Rwork | 0.159 |
| R-free | 0.17930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nm6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.963 |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.730 | 1.670 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Number of reflections | 32308 | |
| <I/σ(I)> | 11.7 | 3.3 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 10.8 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.2 M magnesium chloride, 0.1 M HEPES pH 7.5 and 25 % (w/v) PEG-3350. |
