4P4U

Nucleotide-free stalkless-MxA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95
Spacegroup name	I 21 21 21
Unit cell lengths	46.330, 77.000, 205.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.200 - 1.900
R-factor	0.2126
Rwork	0.210
R-free	0.25909
RMSD bond length	0.009
RMSD bond angle	1.358
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.200	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	29671
<I/σ(I)>	24.6	1.8
Completeness [%]	99.7	95.1
Redundancy	6.9	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.1	291	2.0-2.5M Ammonium sulfate, 0.2M AMPSO/KOH pH9.1