4P4T
GDP-bound stalkless-MxA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 47.020, 77.590, 212.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.050 - 2.300 |
| R-factor | 0.21831 |
| Rwork | 0.216 |
| R-free | 0.25378 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p4u |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.128 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.050 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 17794 | |
| <I/σ(I)> | 28.9 | 2.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.1 | 291 | 2.0-2.5M Ammonium sulfate, 0.2M AMPSO/KOH pH 9.1 |
