4P4Q
Complex of yeast cytochrome c peroxidase (W191F) with iso-1 cytochrome c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.797, 113.842, 88.239 |
| Unit cell angles | 90.00, 105.31, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.010 |
| R-factor | 0.2181 |
| Rwork | 0.216 |
| R-free | 0.27760 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.174 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.432 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.220 | 0.480 |
| Number of reflections | 51802 | |
| <I/σ(I)> | 6.45 | 3.81 |
| Completeness [%] | 91.3 | 82.5 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | PEG 3350 15-15%, 100 mM NaAcetate, 175 mM NaCl |
