4P49

The structure of a chicken anti-prostate specific antigen scFv

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.953697
Spacegroup name	P 61 2 2
Unit cell lengths	52.459, 52.459, 302.375
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.396 - 1.400
R-factor	0.1845
Rwork	0.183
R-free	0.21380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.021
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.396	151.188	1.480
High resolution limit [Å]	1.400	4.430	1.400
Rmerge	0.126	0.079	0.910
Rmeas	0.129
Rpim	0.029	0.019	0.313
Total number of observations	887042	33636	62140
Number of reflections	50126
<I/σ(I)>	12.9	24.7	2.3
Completeness [%]	99.5	100	96.3
Redundancy	17.7	17.4	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.1M NaOAc pH 4.6, 0.2M NH4-sulfate