4P48

The structure of a chicken anti-cardiac Troponin I scFv

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.953689
Spacegroup name	P 21 21 21
Unit cell lengths	51.780, 58.379, 69.048
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.787 - 1.350
R-factor	0.157
Rwork	0.156
R-free	0.17590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.270
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.900	1.420
High resolution limit [Å]	1.350	1.350
Rmerge	0.085
Number of reflections	46638
<I/σ(I)>	14.57	4.2
Completeness [%]	100.0	99.8
Redundancy	7.9	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.6	293.15	0.1M Tris-HCl pH9.6, 0.2M NaOAc, 29% (w/v) PEG 4000 with 0.1M glycine