4P43

Crystal structure of the bacterial A1408U-mutant ribosomal decoding site (C2 form 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	86.450, 31.690, 49.410
Unit cell angles	90.00, 116.34, 90.00

Refinement procedure

Resolution	44.300 - 2.000
Rwork	0.246
R-free	0.27000
RMSD bond length	0.004
RMSD bond angle	0.786
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHENIX
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.280	44.280	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.072	0.040	0.371
Total number of observations	29620	2844	2788
Number of reflections	8251
<I/σ(I)>	7.6	19.8	2.7
Completeness [%]	99.2	96	97.5
Redundancy	3.56	3.27	3.46

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Sodium Cacodylate, Spermine tetrahydrochloride, MPD, Calcium Chloride