4P3S

Crystal structure of the bacterial A1408C-mutant ribosomal decoding site in complex with geneticin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-11
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.91938, 0.92004, 0.90659, 1.0
Spacegroup name	P 21 21 2
Unit cell lengths	34.090, 88.830, 46.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 2.300
Rwork	0.219
R-free	0.26460
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	0.910
Data scaling software	d*TREK
Phasing software	PHENIX
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.420	44.420	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.050	0.023	0.347
Total number of observations	45316	4436	4663
Number of reflections	6694
<I/σ(I)>	17.6	49.2	4.4
Completeness [%]	99.7	97.6	100
Redundancy	6.72	6.05	7.04

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Sodium Cacodylate (pH7), Spermine tetrahydrochloride, MPD, Sodium Chloride