4P3M

Crystal structure of serine hydroxymethyltransferase from Psychromonas ingrahamii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-09-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	146.820, 64.860, 116.940
Unit cell angles	90.00, 125.49, 90.00

Refinement procedure

Resolution	47.609 - 1.850
R-factor	0.1703
Rwork	0.169
R-free	0.21230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gbx
RMSD bond length	0.015
RMSD bond angle	1.514
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.610	1.900
High resolution limit [Å]	1.850	1.850
Number of reflections	76583
<I/σ(I)>	7.3	0.96
Completeness [%]	99.7	99.8
Redundancy	6.8	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	2 M AMMONIUM SULFATE, 150 mM SODIUM CHLORIDE, 100 mM SODIUM CACODYLATE pH 6.5