4P3K
Structure of ancestral PyrR protein (PLUMPyrR)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-07 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.960, 57.240, 54.000 |
| Unit cell angles | 90.00, 127.47, 90.00 |
Refinement procedure
| Resolution | 42.860 - 1.700 |
| R-factor | 0.16669 |
| Rwork | 0.164 |
| R-free | 0.21240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.940 |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.860 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 20089 |
| <I/σ(I)> | 11.5 |
| Completeness [%] | 99.0 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 40% PEG-300, 0.1M Tris pH 7.0, 5% PEG-1000 |
