4P3E

Structure of the human SRP S domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	C 1 2 1
Unit cell lengths	125.649, 70.530, 106.493
Unit cell angles	90.00, 100.46, 90.00

Refinement procedure

Resolution	44.089 - 3.500
R-factor	0.2456
Rwork	0.244
R-free	0.28510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4P3F and 3KTV
RMSD bond length	0.005
RMSD bond angle	1.024
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.090	3.850
High resolution limit [Å]	3.500	3.500
Rmerge		0.360
Number of reflections	11703
<I/σ(I)>	9
Completeness [%]	99.5	99.5
Redundancy	3.4	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	291	Ammonium sulfate, Sodium malonate