4P3D

MT1-MMP:Fab complex (Form II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-03
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.87260
Spacegroup name	P 1 21 1
Unit cell lengths	52.898, 96.226, 81.521
Unit cell angles	90.00, 92.36, 90.00

Refinement procedure

Resolution	39.657 - 1.949
R-factor	0.161
Rwork	0.158
R-free	0.20960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yec
RMSD bond length	0.009
RMSD bond angle	1.187
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1603))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.980
High resolution limit [Å]	1.949	5.290	1.950
Rmerge	0.098	0.047	0.380
Total number of observations	365316
Number of reflections	59340
<I/σ(I)>	8.5
Completeness [%]	99.8	99.9	95.8
Redundancy	6.2	6.1	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	25% PEG 3350, 0.2M MgCl.hexahydrate, 0.1M Hepes