4P37
Crystal structure of the Megavirus polyadenylate synthase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 105 |
| Detector technology | PIXEL |
| Collection date | 2011-10-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793, 0.9795, 0.9770 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.689, 96.132, 153.888 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.380 - 2.240 |
| R-factor | 0.1887 |
| Rwork | 0.187 |
| R-free | 0.21450 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.010 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.300 | 2.360 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.069 | |
| Number of reflections | 62550 | |
| <I/σ(I)> | 6.7 | 1.5 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 3.9 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 0.1M Tris-HCl, PEG 200 |
