4P37

Crystal structure of the Megavirus polyadenylate synthase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	105
Detector technology	PIXEL
Collection date	2011-10-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793, 0.9795, 0.9770
Spacegroup name	P 21 21 21
Unit cell lengths	86.689, 96.132, 153.888
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.380 - 2.240
R-factor	0.1887
Rwork	0.187
R-free	0.21450
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.010
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.300	2.360
High resolution limit [Å]	2.240	2.240
Rmerge	0.069
Number of reflections	62550
<I/σ(I)>	6.7	1.5
Completeness [%]	99.0	99
Redundancy	3.9	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	0.1M Tris-HCl, PEG 200