4P2R
Crystal structure of the 5cc7 TCR in complex with 5c1/I-Ek
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 261.601, 101.867, 214.642 |
| Unit cell angles | 90.00, 94.89, 90.00 |
Refinement procedure
| Resolution | 39.454 - 3.295 |
| R-factor | 0.2133 |
| Rwork | 0.212 |
| R-free | 0.24310 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.816 |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.810 | 3.360 |
| High resolution limit [Å] | 3.290 | 3.290 |
| Rmerge | 1.356 | |
| Number of reflections | 84239 | |
| <I/σ(I)> | 10.9 | 1.3 |
| Completeness [%] | 98.8 | 84.9 |
| Redundancy | 6.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2M Potassium citrate, 18% PEG 3350, 4% 1,3 butanediol |
