4P2J

Crystal structure of the mouse SNX19 PX domain with bound sulphate ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2013
Detector	ADSC QUANTUM 210r
Spacegroup name	P 1 21 1
Unit cell lengths	64.172, 32.987, 82.509
Unit cell angles	90.00, 107.25, 90.00

Refinement procedure

Resolution	61.287 - 2.400
R-factor	0.2208
Rwork	0.219
R-free	0.26340
RMSD bond length	0.008
RMSD bond angle	1.012
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.800
High resolution limit [Å]	2.400
Rmerge	0.097
Number of reflections	13235
<I/σ(I)>	13.4	3.4
Completeness [%]	99.4
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	20% PEG3350, 0.25 M NaCl, 5% glycerol, 0.1 M Tris (pH 8.0)