4P2G
Crystal structure of DJ-1 in sulfinic acid form (aged crystal)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 74.760, 74.760, 75.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.700 - 1.350 |
| R-factor | 0.09852 |
| Rwork | 0.098 |
| R-free | 0.12012 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1soa |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.073 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.740 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.095 | 0.313 |
| Number of reflections | 53628 | |
| <I/σ(I)> | 13.2 | 5.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 200 mM sodium citrate 30% (v:v) PEG400 |
