4P2B
Crystal structure of the apo form of the glutaminyl-tRNA synthetase catalytic domain from Toxoplasma gondii.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.976250 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 105.130, 105.130, 229.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.600 - 2.800 |
| R-factor | 0.2266 |
| Rwork | 0.225 |
| R-free | 0.25580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qtq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.368 |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.600 | 2.920 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.109 | 1.628 |
| Number of reflections | 36436 | |
| <I/σ(I)> | 10.4 | 1.3 |
| Completeness [%] | 98.3 | 98.4 |
| Redundancy | 7.3 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 1 ul 40 g/L protein to 1 ul 2M ammonium sulphate over 2M ammonium sulphate reservoir. |
