4P2A

Structure of mouse VPS26A bound to rat SNX27 PDZ domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-14
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.008
Spacegroup name	P 3 1 2
Unit cell lengths	113.810, 113.810, 94.771
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.905 - 2.700
R-factor	0.2193
Rwork	0.215
R-free	0.25850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 3DQO and 2FAU.
RMSD bond length	0.011
RMSD bond angle	1.357
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.300
High resolution limit [Å]	2.700
Rmerge	0.105	0.964
Number of reflections	19434
<I/σ(I)>	14.8	2.8
Completeness [%]	100.0
Redundancy	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	4 M NaCl, 0.1 M Na Citrate pH 6.0