4P27
Structure of Schistosoma mansoni venom allergen-like protein 4 (SmVAL4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-12-19 |
| Detector | RIGAKU RAXIS HTC |
| Spacegroup name | P 61 |
| Unit cell lengths | 78.645, 78.645, 83.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.630 - 2.160 |
| R-factor | 0.18188 |
| Rwork | 0.180 |
| R-free | 0.20877 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.253 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.050 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.760 | |
| Number of reflections | 15770 | |
| <I/σ(I)> | 5.1 | 1 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 21.2 | 21.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.085 M sodium acetate trihydrate at pH 4.6, 25% (w/v) PEG 2000, 0.17 M ammonium sulfate and 15% (v/v) glycerol, 8 mg/ml SmVAL4 in Sodium Citrate at pH 5.0 |
