4P26
Structure of the P domain from a GI.7 Norovirus variant in complex with A-type 2 HBGA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2013-01-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.580, 63.260, 90.430 |
| Unit cell angles | 99.25, 97.82, 119.62 |
Refinement procedure
| Resolution | 34.024 - 1.900 |
| R-factor | 0.1612 |
| Rwork | 0.160 |
| R-free | 0.18900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p12 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.315 |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.024 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 87902 |
| <I/σ(I)> | 9.1 |
| Completeness [%] | 96.8 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2M Sodium Formate 0.1M Bis Tris Propane 20% PEG3350 |
