4P22

Crystal Structure of N-terminal Fragments of E1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-25
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.975
Spacegroup name	C 2 2 21
Unit cell lengths	94.126, 186.812, 135.222
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.770 - 2.750
R-factor	0.208
Rwork	0.205
R-free	0.26400
RMSD bond length	0.010
RMSD bond angle	1.370
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.748
Number of reflections	30860
<I/σ(I)>	11.3
Completeness [%]	98.4
Redundancy	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.1 M Na3Citrate pH 5.6, 3.0 M NH4Ac