4P20

Crystal structures of the bacterial ribosomal decoding site complexed with amikacin

Replaces:  2G5Q

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-26
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9357
Spacegroup name	P 1 21 1
Unit cell lengths	47.550, 32.820, 52.660
Unit cell angles	90.00, 108.71, 90.00

Refinement procedure

Resolution	10.000 - 2.700
Rwork	0.213
R-free	0.26790
Structure solution method	MOLECULAR REPLACEMENT
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.820	32.820	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.160	0.132	0.432
Total number of observations	15897	1555	1607
Number of reflections	4398
<I/σ(I)>	4.8	7.9	2
Completeness [%]	100.0	100	100
Redundancy	3.59	3.25	3.61

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.4	310	MPF, Na Cacodylate, NaCl, KCl