4P1Q

GREEN FLUORESCENT PROTEIN E222H VARIANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97620
Spacegroup name	P 21 21 21
Unit cell lengths	50.600, 62.200, 67.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.760 - 1.500
R-factor	0.13802
Rwork	0.136
R-free	0.17228
RMSD bond length	0.024
RMSD bond angle	2.362
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	33.810
High resolution limit [Å]	1.500
Number of reflections	34827
<I/σ(I)>	6.3
Completeness [%]	100.0
Redundancy	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Na-acetate, PEG 3350