4P1O

Crystal structure of the Bateman domain of murine magnesium transporter CNNM2 bound to ATP-Mg

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR-H
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-12-11
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54179
Spacegroup name	P 43 21 2
Unit cell lengths	104.633, 104.633, 99.861
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.080 - 3.060
R-factor	0.224
Rwork	0.221
R-free	0.25520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.801
Data scaling software	HKL-2000
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	33.080
High resolution limit [Å]	3.060
Number of reflections	10982
<I/σ(I)>	12.43
Completeness [%]	96.0
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Sodium Acetate, 3.5M Ammonium Acetate, 5mM Magnesium chloride, 10mM ATP