4P1G

Crystal structure of the Bateman domain of murine magnesium transporter CNNM2 bound to AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	I 41 2 2
Unit cell lengths	105.579, 105.579, 102.202
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.150 - 2.603
R-factor	0.2159
Rwork	0.212
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.153
Data scaling software	XDS
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	28.150
High resolution limit [Å]	2.600
Number of reflections	8072
<I/σ(I)>	53.58
Completeness [%]	99.6
Redundancy	13.71

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.1 M Na acetate pH 4.6, 3 M ammonium acetate, 10 mM AMP