4P17

Crystal structure of the Chlamydomonas flagellar RabGAP TBC domain.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-10-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9788
Spacegroup name	C 1 2 1
Unit cell lengths	121.760, 47.010, 105.670
Unit cell angles	90.00, 96.33, 90.00

Refinement procedure

Resolution	60.509 - 1.794
R-factor	0.1965
Rwork	0.195
R-free	0.25960
RMSD bond length	0.011
RMSD bond angle	1.311
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	60.510
High resolution limit [Å]	1.790
Number of reflections	55508
<I/σ(I)>	5.2
Completeness [%]	99.0
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	295	50mM MES pH 6 0.2M NaCl 18% PEG 8000