4P12
Native Structure of the P domain from a GI.7 Norovirus variant.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-12-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.181, 63.179, 90.470 |
| Unit cell angles | 99.51, 97.89, 119.17 |
Refinement procedure
| Resolution | 39.018 - 1.601 |
| R-factor | 0.1568 |
| Rwork | 0.155 |
| R-free | 0.18170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zl5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.241 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.096 | 0.650 |
| Number of reflections | 146576 | |
| <I/σ(I)> | 14.1 | 2.2 |
| Completeness [%] | 97.1 | 95.3 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2M Sodium Formate, 0.1M Bis Tris Propane, 20% PEG 3350 |
