4P11
Native crystal structure of MltF Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-10-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.258, 82.473, 96.921 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.781 - 1.891 |
| R-factor | 0.1524 |
| Rwork | 0.151 |
| R-free | 0.18520 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.851 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.460 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.103 | |
| Rpim | 0.044 | |
| Total number of observations | 209480 | |
| Number of reflections | 43654 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 99.4 | 99.1 |
| Redundancy | 4.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1 M Tris pH 8.0, 0.2 M MgCl2, 20% (w/v) PEG8000 |
