4P0Z
Structure of the double stranded DNA binding type IV secretion protein TraN from Enterococcus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.150, 55.090, 35.520 |
| Unit cell angles | 90.00, 113.05, 90.00 |
Refinement procedure
| Resolution | 32.680 - 1.350 |
| R-factor | 0.17 |
| Rwork | 0.169 |
| R-free | 0.20200 |
| Structure solution method | SAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.481 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.684 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.629 | |
| Number of reflections | 25731 | |
| <I/σ(I)> | 10.9 | 1.2 |
| Completeness [%] | 99.4 | 98.6 |
| Redundancy | 7.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 298 | BIS-TRIS, PEG 3.350, SEEDING STOCK 1:1 |
