4P0X

Human farnesyl diphosphate synthase in complex with Taxodione

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	298
Detector technology	CCD
Collection date	2013-12-13
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 41 21 2
Unit cell lengths	110.623, 110.623, 78.443
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.310 - 2.500
R-factor	0.2109
Rwork	0.207
R-free	0.27910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.760
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	55.310
High resolution limit [Å]	2.300
Number of reflections	22231
<I/σ(I)>	44.04
Completeness [%]	99.8
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	1.2M sodium potassium phosphate, 25% glycerol, 1mM GPP