4P0K
Crystal Structure of Double Loop-Swapped Interleukin-36Ra
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 43.115, 43.115, 119.122 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.339 - 1.700 |
| R-factor | 0.1968 |
| Rwork | 0.195 |
| R-free | 0.23270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1md6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.076 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.340 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.091 | 0.837 |
| Rpim | 0.034 | 0.331 |
| Total number of observations | 117020 | 5540 |
| Number of reflections | 14865 | |
| <I/σ(I)> | 14.8 | 2.2 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 7.9 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 298 | 25% (w/v) PEG5000MME, 0.1 M NaAcetate, pH 5.3 |
