4P0G
Crystal structure of MltF from Pseudomonas aeruginosa complexed with bulgecin and muropeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-11-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.325, 82.239, 95.207 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.575 - 1.650 |
| R-factor | 0.1617 |
| Rwork | 0.160 |
| R-free | 0.18710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.009 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.575 | 47.575 | 1.740 |
| High resolution limit [Å] | 1.650 | 5.220 | 1.650 |
| Rmerge | 0.020 | 0.626 | |
| Rmeas | 0.073 | ||
| Rpim | 0.036 | 0.012 | 0.361 |
| Total number of observations | 229815 | 7586 | 30989 |
| Number of reflections | 55809 | ||
| <I/σ(I)> | 17.2 | 46.8 | 2.3 |
| Completeness [%] | 99.8 | 99.9 | 98.7 |
| Redundancy | 4.1 | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1 M Tris pH 8.0, 0.2 M MgCl2, 20% (w/v) PEG8000 |
