4OZX
Crystal Structure of the alginate lyase from Klebsiella pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 95.299, 95.299, 67.218 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.649 - 1.880 |
R-factor | 0.1791 |
Rwork | 0.177 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.067 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.089 | 0.621 |
Number of reflections | 28939 | |
<I/σ(I)> | 24.74 | |
Completeness [%] | 99.9 | |
Redundancy | 24 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% (w/v) PEG 8000, 0.1 M HEPES pH 7.5 |