4OZS
RNA binding protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.980, 0.954 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.025, 75.023, 85.117 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.280 - 2.170 |
| R-factor | 0.21933 |
| Rwork | 0.217 |
| R-free | 0.26549 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.442 |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.280 | 2.250 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.019 | 0.313 |
| Number of reflections | 18791 | |
| <I/σ(I)> | 24.44 | 2.68 |
| Completeness [%] | 99.8 | 98.43 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.35 | 293 | Diffraction quality crystals grew from 3 uL drops in 24-well sitting drop Cryschem plates (Hampton Research) in 2:1 ratios of crystallant 100 mM Sodium citrate pH 3.35, 8 % (w/v) PEG 3350 and protein equilibrated against 1 mL of crystallant at 293 K. |
