4OZR

Crystal structure of the ligand binding domains of the Bovicola ovis ecdysone receptor EcR/USP heterodimer (methylene lactam crystal)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9536
Spacegroup name	C 1 2 1
Unit cell lengths	153.671, 42.430, 86.669
Unit cell angles	90.00, 117.70, 90.00

Refinement procedure

Resolution	68.030 - 2.700
R-factor	0.2256
Rwork	0.224
R-free	0.25850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.134
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	69.300
High resolution limit [Å]	2.700
Number of reflections	13311
<I/σ(I)>	9.1
Completeness [%]	96.2
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	50 mM Tris pH 7.5, 150 mM NaCl, 5% glycerol, 5 mM DTT, 0.02% azide, 0.37 mM methylene lactam