4OZL

GlnK2 from Haloferax mediterranei complexed with AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-27
Detector	PSI PILATUS 6M
Wavelength(s)	0.9618
Spacegroup name	H 3 2
Unit cell lengths	89.090, 89.090, 95.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.956 - 1.494
R-factor	0.1625
Rwork	0.161
R-free	0.18420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.162
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	59.960
High resolution limit [Å]	1.020
Number of reflections	23723
<I/σ(I)>	37.3
Completeness [%]	100.0
Redundancy	19.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	294	2 M ammonium sulphate, 0.1 M NaCitrate, 0.2 M Na/K Tartrate