4OZH
S16 protein complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 261.569, 57.870, 137.411 |
| Unit cell angles | 90.00, 114.05, 90.00 |
Refinement procedure
| Resolution | 40.990 - 2.800 |
| R-factor | 0.2273 |
| Rwork | 0.226 |
| R-free | 0.24290 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.890 |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.990 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.049 |
| Number of reflections | 43700 |
| <I/σ(I)> | 2 |
| Completeness [%] | 93.2 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Mother liquor: 0.15- 0.25M CaOAc, 0.1 M Tris/HCl, 12-18% PEG3350, Additives: 2-3 mM reduced and oxidised Glutathione |
