4OZF

JR5.1 protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95370
Spacegroup name	C 1 2 1
Unit cell lengths	130.930, 84.840, 109.680
Unit cell angles	90.00, 92.99, 90.00

Refinement procedure

Resolution	60.470 - 2.700
R-factor	0.1864
Rwork	0.184
R-free	0.23840
RMSD bond length	0.009
RMSD bond angle	1.140
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	60.470
High resolution limit [Å]	2.700
Rmerge	0.106
Number of reflections	32383
<I/σ(I)>	5.87
Completeness [%]	97.8
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Mother liquor: 0.15 - 0.25M CaOAc, 0.1M Tris/HCl, 12-18% PEG3350, Additives:2-3 mM reduced and oxidised Glutathione